Tymola test norm and diagnostic value

In a number of diseases, the setting of sediment samples is used for the diagnosis. One of them is the timole test, proposed in 1944 by McLagan. It is based on the change in the colloidal resistance of serum proteins in diseases accompanied by dysproteinemia.

Normally, blood proteins are in a state of high stability. When the ratio of fractions of albumins and globulins changes, the colloidal resistance of proteins decreases. The lower it is, the more proteins precipitate and precipitate when the thymol reagent is added.

When carrying out the sample as a reagent, use an alcohol solution of thymol in veronal buffer or tris buffer. Finally, the reaction chemistry is not clear. However, thymol assay is clearly correlated with the clinical picture of diseases occurring with dysproteinemia. It is easy to set up and labor-consuming, so it continues to be widely used in biochemical blood tests.

When the sample is put into saline, the serum of the patient is added, then the reagent is added. If in norm the thymol probe is accompanied by a very slight loss of protein flakes and a slight cloudiness of the reaction mixture, then in the case of disproteinemia the solution becomes significantly turbid. The degree of turbidity depends on the degree of disturbance of the colloidal properties of proteins. The result of the sample increases with a decrease in albumins and an increase in beta and gamma globulins.

The degree of turbidity is measured on a biochemical analyzer or photoelectric colorimeter. As a reference solution, barium chloride is used in a certain concentration.

This study has special clinical significance for hepatitis, collagenosis and other diseases, accompanied by disproteinemia - a violation of the ratio of whey proteins. For hepatic damage in hepatitis is characterized by an elevated thymol test. The norm is from 0 to 4 units. When hepatitis it becomes positive a week before jaundice. In some cases, there is an increase in the sample to 20 or more units. At such high rates it is necessary to repeat the test with a serum diluted 1: 1 and increase the result by 2 times.

For setting the sample, hemolyzed serum is not suitable. When hemolysis, the destruction of erythrocytes, it is painted red. In this case, the sample will be overestimated. The analysis should be repeated after a new blood collection from the vein.

The thymol sample is overestimated if the serum is lipemic (chyle), cloudy because of the presence of lipids (chylomicra) in it. The laboratory assistant, when conducting a test with such serum, should, in lieu of control over saline solution, take control of the patient's serum diluted with saline.

To avoid futility, blood for biochemical research should be taken strictly on an empty stomach. It should be delivered to the laboratory no later than 2 hours after blood sampling. When stored in a refrigerator, the serum is suitable for setting the sample for not more than 7 days.

What does elevated thymol tryout mean? Its interpretation is as follows: if it significantly exceeds the norm, one can think of liver disease (hepatitis or cirrhosis), a kidney disease that occurs with a nephrotic syndrome, systemic diseases such as rheumatism, rheumatoid polyarthritis, scleroderma. One indicator can not be diagnosed. Timolovy trial should be evaluated in conjunction with other studies. If a liver disease is suspected, at the same time, biochemical analyzes should be made at the level of total and direct bilirubin, transaminase, cholesterol, alkaline phosphatase, zinc sulfate or sulphate test.

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